Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human RAD51 around the phosphorylation site of threonina 309(G-E-TP-R-I).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q06609
Applications:
IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
DNA repair protein RAD51 homolog 1; RAD51A; RECA
Specificity Sensitivity:
RAD51 (Phospho-Thr309) antibody detects endogenous levels of RAD51 only when phosphorylated at threonina 309.
References:
Shinohara A., Nat. Genet. 4:239-243(1993).
Yoshimura Y., Nucleic Acids Res. 21:1665-1665(1993).
Schmutte C., Cancer Res. 59:4564-4569(1999).
All Sites:
Human: Thr309; Mouse: Thr309
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAD51 (Phospho-Thr309) antibody.