• Catalog Number:

  E011191-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q92769

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

• Specificity Sensitivity:

  HDAC2 (Phospho-Ser394) antibody detects endogenous levels of HDAC2 only when phosphorylated at serine 394.

• References:

  Tsai SC, et al.(2002)J Biol Chem; 277(35): 31826-33

Western blot analysis of extracts from HT-29 cells untreated or treated with UV (20min), using HDAC2 (Ab-394) antibody (E021140, Lane 1 and 2) and HDAC2 (Phospho-Ser394) antibody (E011191, Lane 3 and 4).

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho- Ser394) antibody (E011191).