• Catalog Number:

  E20-60931

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human CDC16/APC6 around the phosphorylation site of Ser560. AA range526-575

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human,Mouse

• Source:

  Rabbit

• Applications:

  WB: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Other Names:

  CDC16; ANAPC6; Cell division cycle protein 16 homolog; Anaphase-promoting complex subunit 6; APC6; CDC16 homolog; CDC16Hs; Cyclosome subunit 6

• Specificity Sensitivity:

  Phospho-Cdc16 (S560) Polyclonal Antibody detects endogenous levels of Cdc16 protein only when phosphorylated at S560.

Immunohistochemical analysis of paraffin- embedded Human brain. Antibody was diluted at 1:100 (4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho- ELISA) for Immunogen Phospho- peptide (left) and Non- Phospho- peptide (right), using CDC16/ APC6 (Phospho-Ser560) Antibody.

WB analysis of lysates from HUVEC cells, using CDC16/APC6 (Phospho-Ser560) Antibody. The lane on the right is blocked with the phospho peptide. Expression: Aorta endothelial cell,Brain,Epithelium,Lung,Skin.