• Catalog Number:

  E011083-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ICMA-1 around the phosphorylation site of tyrosine 512 (K-K-YP-R-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05362

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  ICAM-1 (Phospho-Tyr512) antibody detects endogenous levels of ICAM-1 only when phosphorylated at tyrosine 512.

• References:

  Greenwood J, et al. (2003) J Immunol; 171(4):2099-2108. Zhou Z, et al. (2005) Eur J Pharmacol; 513(1-2):1-8. Chen YH, et al. (2001) J Cell Biochem; 82(3):512-521

Western blot analysis of extracts from HUVEC cells, using ICAM-1 (Ab-512) Antibody (E021106, Lane 1, 2 and 3) and ICAM-1 (Phospho-Tyr512) Antibody (E011083, Lane 4, 5 and 6).