• Catalog Number:

  E20-60544

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453. AA range421-470

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human,Mouse,Rat

• Source:

  Rabbit

• Applications:

  WB: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Other Names:

  CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD antigen CD5

• Specificity Sensitivity:

  Phospho-CD5 (Y453) Polyclonal Antibody detects endogenous levels of CD5 protein only when phosphorylated at Y453.

WB analysis of 293 cells using Phospho-CD5 (Y453) Polyclonal Antibody

Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.

Enzyme-Linked Immunosorbent Assay (Phospho- ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non- Phosphopeptide (Phospho- right), using CD5 (Phospho- Tyr453) Antibody