• Catalog Number:

  E1A8281A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of tyrosine 324 (G-D-YP-G-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P98172/P52799/Q15768

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  CEK5 ligand; CEK5 receptor ligand; CEK5-L; CEL5-L; EFL-3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; Ephrin-B1 precursor; EPL2; EPLG2; kinase ephrin-B1; LERK-2; LERK2; STRA1; STRA1 protein

• Specificity Sensitivity:

  Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.

• References:

  Beckmann M.P., EMBO J. 13:3757-3762(1994). Davis S., Science 266:816-819(1994). Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Tyr324/311/318; Mouse: Tyr323/314/318; Rat: Tyr323/-/318

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody. Western blot analysis of extracts from K562 cells, treated with serum (20%, 15mins), using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody.