• Catalog Number:

  E011014-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  NF-κB p65 (phospho-Ser536) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 536.

• References:

  Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501. Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p65 (phospho-Ser311) antibody (E011260).

Western blot analysis of extract from Hela cells untreated or treated with IFN, using NFκB-p65 (Ab-311) antibody (E021252, Lane 1 and 2) and NFκB-p65 (phospho-Ser311) antibody (E011260, Lane 3 and 4).

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (phospho-Thr254) antibody (E011010).