• Catalog Number:

  E012070-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Crk2 around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q64010

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  Crk2 ( Phospho-Tyr221 ) antibody detects endogenous levels of Crk2 only when phosphorylated at tyrosine 221.

• References:

  Ogawa S, et al. (1994) Oncogene 9:1669-1678. Abassi Y.A., et al. (2002) EMBO J. 21:4571-4582. Feller S.M., et al. (1994) EMBO J. 13:2341-2351. Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Crk2 (Phospho-Try221) Antibody (E012070).