• Catalog Number:

  E1A7238A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 235 (P-K-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10636

• Applications:

  WB: 1:500~1000 ELISA: 1:20000

• Alter Name:

  MAPT, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

• Specificity Sensitivity:

  Tau (phospho-Ser235) antibody detects endogenous levels of Tau only when phosphorylated at serine 235.

• References:

  Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881. Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214. Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309. Kyriakis J M, et al. (1994) Nature. 369: 156-160.

• All Sites:

  Human: Ser235; Mouse: Ser527; Rat: Ser546

Western blot analysis of extracts from mouse brain tissue using Tau (Phospho-Ser235) Antibody.