Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Synuclein-α around the phosphorylation site of tyrosine 125 (E-A-YP-E-M).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P37840
Applications:
IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; NACP; PARK1
Specificity Sensitivity:
Synuclein-α (Phospho-Tyr125) antibody detects endogenous levels of Synuclein-α only when phosphorylated at tyrosine 125.
References:
Ueda K., Proc. Natl. Acad. Sci. U.S.A. 90:11282-11286(1993).
Campion D., Genomics 26:254-257(1995).
Ueda K., Biochem. Biophys. Res. Commun. 205:1366-1372(1994).
All Sites:
Human: Tyr125; Mouse: Tyr125; Rat: Tyr125
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Synuclein-α (Phospho-Tyr125) antibody.