Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CDK6 around the phosphorylation site of tyrosine 13 (Q-Q-YP-E-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q00534
Applications:
IHC: 1:50~100 IF:1:100~1:200
Specificity Sensitivity:
CDK6 (phospho-Tyr13) Antibody detects endogenous levels of CDK6 only when phosphorylated at tyrosine 13.
References:
Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using CDK6 (phospho-Tyr13) Antibody (E011542).
Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr13) Antibody (E011542, Red).