• Catalog Number:

  E1A7101B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC5 around the phosphorylation site of Serine 498.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UQL6

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  HDAC5 (Ab-498) Antibody detects endogenous levels of total HDAC5 protein.

• References:

  Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019. McKinsey TA, et al. (2000) Nature. 408(6808): 106-111.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using HDAC5 (Ab-498) Antibody (#B7101). Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis of the extracts from NIH/3T3 cells using HDAC5 (Ab-498) Antibody (#B7101) and HDAC5 (Phospho-Ser498) Antibody (#11193): Line1: Using #B7101; Line2: Using #B7101 preincubated with synthesized peptide.