• Catalog Number:

  E1A8032A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of serine 843 (R-G-SP-I-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.10.2; FADK 1; FAK1; Focal adhesion kinase 1; pp125FAK; Protein- tyrosine kinase 2; PTK2

• Specificity Sensitivity:

  FAK (Phospho-Ser843) antibody detects endogenous levels of FAK only when phosphorylated at serine 843.

• References:

  Whitney G.S., DNA Cell Biol. 12:823-830(1993). Andre E., Biochem. Biophys. Res. Commun. 190:140-147(1993). Lee S.-T., Oncogene 8:3403-3410(1993).

• All Sites:

  Human: Ser843; Mouse: Ser881; Rat: Ser843

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 20mins), using FAK (Phospho-Ser843) antibody.