Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
chk2 around the phosphorylation site of Serine 33 (S-S-SP-Q-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
O96017
Applications:
IHC: 1:50~100
Specificity Sensitivity:
chk2 (phospho-Ser33) Antibody detects endogenous levels of chk2 only when
phosphorylated at Serine 33.
References:
Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using chk2 (phospho-Ser33) Antibody (E011527).