• Catalog Number:

  E1A7192A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49023

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  Paxillin (phospho-Tyr118) antibody detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.

• References:

  Tao WA, et al.(2005)Nat Methods.2(8): 591-598. Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250. Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522. Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.

Western blot analysis of extracts from HeLa cells using Paxillin (Ab-118) antibody (line 1 and 2) and Paxillin (phospho- Tyr118) antibody (line 3 and 4).