• Catalog Number:

  E1A7156A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42345

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  FRAP, FRAP1, FRAP2, RAFT2, Rapamycin target protein, kinase mTOR

• Specificity Sensitivity:

  mTOR (phospho-Ser2448) antibody detects endogenous levels of mTOR only when phosphorylated at serine 2448.

• References:

  herine M. Hannan, Mol. Cell. Biol., Dec 2003; 23: 8862 - 8877. Dos D. Sarbassov, Science, Feb 2005; 307: 1098 - 1101. Susan W. Y. Cheng, J. Biol. Chem., Apr 2004; 279: 15719 - 15722. Charles H. Lang, Am J Physiol Endocrinol Metab, Dec 2003; 285: E1205 - E1215.

• All Sites:

  Human: Ser2448; Mouse: Ser2448; Rat: Ser2448

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using mTOR (phospho-Ser2448) antibody.

Western blot analysis of extracts from Hela cells treated with EGF (200ng/ml, 30mins), using mTOR (phospho-Ser2448) (Line 1 and 2).