• Catalog Number:

  E011254-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of threonine 421 (P-R-TP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  p70 S6 Kinase (phospho-Ser421) antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at threonine 421.

• References:

  Xiao-Feng, et al. (2003) Le1 30 Volume 22: 484-497 An WL, et al. (2003) Am J Pathol. 163(2): 591-607. Le XF, et al. (2003) Oncogene.22(4): 484-97

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (phospho-Thr421) antibody (E011254).

Western blot analysis of extracts from Jurkat cells, untreated or treated with GM-CSF (25ng/ml 30min) using p70 S6 Kinase (phospho-Thr421) antibody (E011254, Line 1 and 2).