• Catalog Number:

  E1A1065A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IL-7R/CD127 around the phosphorylation site of tyrosine 449 (E-A-YP-V-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P16871

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CD127 antigen, CDw127, IL-7R alpha, IL7RA, interleukin-7 receptor alpha chain

• Specificity Sensitivity:

  IL-7R/CD127 (Phospho-Tyr449) antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at tyrosine 449.

• References:

  D Benjamin, J. Immunol., May 1994; 152: 4749. TH Page, J. Immunol., Nov 1993; 151: 4753. Kohtaro Fujihashi, PNAS, Apr 1996; 93: 3613.

• All Sites:

  Human: Tyr449; Mouse: Tyr449

P-peptide - + Western blot analysis of extracts from HeLa cells, using IL-7R/CD127 (Phospho-Tyr449) antibody. Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) antibody.