• Catalog Number:

  E011235-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 307 (T-E-SP-I-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  IRS-1 (phospho-Ser307) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 307.

• References:

  Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575 Tuncman G, et al. (2006) Proc Natl Acad Sci U S A; 103(28): 10741-10746 Shoelson SE, et al. (2006) J Clin Invest; 116(7): 1793-1801

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (phospho-Ser307) antibody (E011235).