• Catalog Number:

  E1A8471A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLD1 around the phosphorylation site of threonine 147.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  Q13393

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Phospholipase D1, PLD 1, hPLD1, EC=3.1.4.4, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1

• Specificity Sensitivity:

  PLD1 (Phospho-Thr147) antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.

• References:

  Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646]. Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774]. Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

• All Sites:

  Human: Thr147; Mouse: Thr147; Rat: Thr147

Western blot analysis of extracts from HepG2 cells, using PLD1 (Phospho-Thr147) antibody.