Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Glutamate receptor 2 (Precursor) around the phosphorylation site of serine 880 (I-E-SP-V-K)(CYNVYGIESVKI).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P42262
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Glutamate receptor 2 (Precursor)(phospho-Ser880) antibody detects endogenous levels of Glutamate receptor 2 (Precursor) only when phosphorylated at serine 880
References:
Cull-Candy, S. et al. (2006) Curr. Opin. Neurobiol. 16, 288-297.
Hanley JG, et al. (2002) Neuron. 34(1): 53-67
Western blot analysis of extract from mouse brain tissue, using Glutamate receptor 2 (Precursor)(Ab-880) antibody (E021284, Lane 1 and 2) and Glutamate receptor 2 (Precursor)(phospho-Ser880) antibody (E011292, Lane 3 and 4).