Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCμ around the phosphorylation site of serine 738 (E-K-SP-F-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15139
Applications:
WB: 1:500~1:1000
Alter Name:
PRKCQ , Protein kinase C theta type
Specificity Sensitivity:
PKD/PKCμ (phospho-Ser738) antibody detects endogenous levels of PKD/PKCμ only when phosphorylated at serine 738.
References:
Storz P, et al. Mol Cell Biol. 2004 Apr; 24(7): 2614-2626.
Storz P, et al. Mol Cell Biol. 2005 Oct; 25(19): 8520-8530.
Zhang W, et al. J Biol Chem 2005 May 13; 280(19): 19036-19044