• Catalog Number:

  E1A8462A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HS1 around the phosphorylation site of tyrosine 397 (G-D-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P14317

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  HCLS1; Hematopoietic cell- specific LYN substrate 1; Hematopoietic lineage cell specific protein; LCKBP1

• Specificity Sensitivity:

  HS1 (Phospho-Tyr397) antibody detects endogenous levels of HS1 only when phosphorylated at tyrosine 397.

• References:

  Kitamura D., Nucleic Acids Res. 17:9367-9379(1989). Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Ota T., Nat. Genet. 36:40-45(2004).

• All Sites:

  Human: Tyr397

Western blot analysis of extracts from Jurkat cells treated with TNF, using HS1 (Phospho-Tyr397) antibody.