• Catalog Number:

  E1A8031A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 478 (P-V-YP-E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P15311

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  Cytovillin; EZRI; p81; VIL2; Villin 2

• Specificity Sensitivity:

  Ezrin (Phospho-Tyr478) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 478.

• References:

  Gould K.L., EMBO J. 8:4133-4142(1989). Turunen O., J. Biol. Chem. 264:16727-16732(1989). Mungall A.J., Nature 425:805-811(2003).

• All Sites:

  Human: Tyr478; Mouse: Tyr478; Rat: Tyr478

Lane 1: The extracts from K562 cells treated with Na3VO4 (0.3nM, 40mins) using Ezrin (Phospho-Tyr478) antibody Lane 2: The extracts from K562 cells treated with Na3VO4 (0.3nM, 40mins) plus phosphopeptide using Ezrin (Phospho-Tyr478) antibody