• Catalog Number:

  E1A8389A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from humanp16-INK4a around the phosphorylation site of serine 152 (G-P-SP-D-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P42771

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  CD2A1; CDK4I; CDKN2; CDKN2A; CDN2; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; MTS1; Multiple tumor suppressor 1; p14ARF; p16(INK4a); p16-INK4; P16INK4A

• Specificity Sensitivity:

  p16-INK4a (Phospho-Ser152) antibody detects endogenous levels of p16-INK4a only when phosphorylated at serine 152.

• References:

  Serrano M., Nature 366:704-707(1993). Robertson K.D., Oncogene 18:3810-3820(1999). Kitagawa Y., J. Biol. Chem. 277:46289-46297(2002).

• All Sites:

  Human: Ser152

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p16-INK4a (Phospho-Ser152) antibody. Western blot analysis of extracts from HeLa cells, treated with EPO (20U/ml, 15mins), using p16-INK4a (Phospho-Ser152) antibody.

p16-INK4a (Phospho-Ser152) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.