Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of threonin 202/185 and tyrosine 204/187.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
ERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) antibody detects endogenous levels of ERK1/2 only when phosphorylated at threonin 202/185 and tyrosine 204/187.