• Catalog Number:

  E1A8376A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Mnk1 around the phosphorylation site of threonine 385 (L-P-TP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9BUB5

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MKNK1

• Specificity Sensitivity:

  Mnk1 (Phospho-Thr385) antibody detects endogenous levels of Mnk1 only when phosphorylated at threonine 385.

• References:

  Fukunaga R., EMBO J. 16:1921-1933(1997). Knauf U., Mol. Cell. Biol. 21:5500-5511(2001). O'Loghlen A., Exp. Cell Res. 299-343-355(2004).

• All Sites:

  Human: Thr385; Mouse: Thr344; Rat: Thr332

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mnk1 (Phospho-Thr385) antibody. Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Phospho-Thr385) antibody.