Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PI3K P85 around the phosphorylation site of tyrosine 467 (L-YP-E-E-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P27986 Q92569 O00459
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
PI3K P85(phospho-Tyr467) antibody detects endogenous levels of PI3K P85 only when phosphorylated at tyrosine 467.
References:
Stoyanov B, et al. (1995) Science;269, 690-3.
Western blot analysis of extract from 293 cells untreated or treated with sorbitol using PI3K P85(phospho-Tyr467) antibody (E011508).