• Catalog Number:

  E1A8221A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TOP2A around the phosphorylation site of threonine 1343 (E-K-TP-D-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P11388

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  alpha isozyme; DNA topoisomerase II; DNA topoisomerase II-alpha; EC 5.99.1.3; P11388-1; TOP-2; TOP2; TOP2A; TP2A

• Specificity Sensitivity:

  TOP2A (Phospho-Thr1343) antibody detects endogenous levels of TOP2A only when phosphorylated at threonine 1343.

• References:

  Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988). Wasserman R.A., Cancer Res. 53:3591-3596(1993). Lang A.J., Gene 221:255-266(1998).

• All Sites:

  Human: Thr1343

Western blot analysis of extracts from HepG2 cells, treated with Ca2+ (40nM, 30mins), using TOP2A (Phospho-Thr1343) antibody.