• Catalog Number:

  E011103-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLCγ1 around the phosphorylation site of tyrosine 783 (G-F-YP-V-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19174

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  PLCγ1 (phospho-Tyr783) antibody detects endogenous levels of PLCγ1 only when phosphorylated at tyrosine 783.

• References:

  DeBell KE, et al. Mol Cell Biol. 1999 Nov; 19(11): 7388-7398. Verí MC, et al. Mol Cell Biol. 2001 Oct; 21(20): 6939-6950. Sette C, et al. EMBO J. 2002 Oct 15; 21(20): 5386-5395.

Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PLCγ1 (Ab-783) antibody (E021129, Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody (E011103, Lane 3 and 4).