• Catalog Number:

  E1A8081A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BLK around the phosphorylation site of tyrosine 501 (R-Q-YP-E-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%);Rat (Identities = 91%, Positives = 91%)

• SwissProtNo:

  P51451

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  B lymphocyte kinase; EC 2.7.10.2; kinase Blk; p55-BLK; Tyrosine-protein kinase BLK

• Specificity Sensitivity:

  BLK (Phospho-Tyr501) antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.

• References:

  Islam K.B., J. Immunol. 154:1265-1272(1995). Drebin J.A.,Oncogene 10:477-486(1995). Greenman C.,Nature 446:153-158(2007).

• All Sites:

  Human: Tyr501; Mouse: Tyr495; Rat: Tyr495

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody. BLK (Phospho-Tyr501) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.