• Catalog Number:

  E1A8161A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PAK2 around the phosphorylation site of serine 141 (Y-L-SP-F-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13177

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  EC 2.7.11.1; gamma-PAK; kinase PAK2; P21-activated kinase 2; p21-activated kinase 2; p21-activated protein kinase I; PAK 2; PAK-2; PAK65; PAKI; S6/H4 kinase

• Specificity Sensitivity:

  PAK2 (Phospho-Ser141) antibody detects endogenous levels of PAK2 only when phosphorylated at serine 141.

• References:

  Sells M., Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004). Martin G.A., EMBO J. 14:1970-1978(1995). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser141; Mouse: Ser141; Rat: Ser141

Western blot analysis of extracts from HepG2 cells treated with Adriamycin (0.5uM, 24hours) and Jurkat cells treated with PMA (125ng/ml, 30mins) using PAK2 (Phospho-Ser141) antibody.