• Catalog Number:

  E011248-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 82 (Q-L-SP-S-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04792

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  HSP27 (phospho-Ser82) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 82.

• References:

  White MY, et al. (2006) J Mol Cell Cardiol. 40(6): 761-74 Xiankui Sun, et al. (2006) Cell Stress Chaperones. 11(1): 61–70 Matthias Meier; et al. (2001) Hypertension. 38:1260 Ali Dana, et al. (2000) Circulation Research. 86: 989

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (phospho-Ser82) antibody (E011248).

Western blot analysis of extracts from Hela cells untreated or treated with Heat Shock or UV, using HSP27 (Ab-82) antibody (E021240, Lane 1, 2 and 3) and HSP27 (Phospho-Ser82) antibody (E011248, Lane 4, 5 and 6).