• Catalog Number:

  E1A7233A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human synapsin around the phosphorylation site of serine 9 (R-L-SP-D-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17600

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  SYN1, Synapsin-1

• Specificity Sensitivity:

  synapsin (phospho-Ser9) antibody detects endogenous levels of synapsin only when phosphorylated at serine 9.The antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms.

• References:

  Diviya Sinha, et,al. (2005) Am J Physiol Renal Physiol; 288: F703-F713. Franco Onofri, et,al. (2000) J. Biol. Chem; 275: 29857. Dario Bonanomi, et,al. (2005) J. Neurosci; 25: 7299-7308. Hiroshi Tokumitsu, et,al. (2005) J. Biol. Chem; 280: 35108-35118.

• All Sites:

  Human: Ser9; Mouse: Ser9; Rat: Ser9

Western blot analysis of extracts from 293 cells untreated or treated with PMA (200nM, 30mins), using synapsin (Ab-9) antibody (Line 1 and 2) and synapsin (phospho-Ser9) antibody (Line 3 and 4).