• Catalog Number:

  E1A1037A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HIRA around the phosphorylation site of threonine 555 (L-T-T P-P-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P54198

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  DGCR1, TUP1 like enhancer of split protein 1, TUPLE1

• Specificity Sensitivity:

  HIRA (Phospho-Thr555) antibody detects endogenous levels of HIRA only when phosphorylated at threonine 555.

• References:

  Lamour V., Hum. Mol. Genet. 4:791-799(1995). Lorain S., Genome Res. 6:43-50(1996). Halford S., Hum. Mol. Genet. 2:2099-2107(1993).

• All Sites:

  Human: Thr555; Mouse: Thr554

P-peptide - + Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) antibody.