• Catalog Number:

  E1A1172A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 359 (Q-R-SP-K-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P14598

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox

• Specificity Sensitivity:

  p47 phox (Phospho-Ser359) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 359.

• References:

  Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989). Lomax K.J., Science 245:409-412(1989).

• All Sites:

  Human: Ser359

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using p47 phox (Phospho-Ser359) antibody. Western blot analysis of extracts from HeLa cells treated with nocodazole (1ug/ml, 18hours) and HeLa cells treated with TSA (400nM, 24hours), using p47 phox (Phospho-Ser359) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) antibody.