Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of serine 780 (R-L-SP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P42229
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000 IP: Various Dilution
Specificity Sensitivity:
STAT5A (phospho-Ser780) antibody detects endogenous levels of STAT5A only when phosphorylated at serine 780.
References:
Gouilleux F, et al. (1994) EMBO J. 13: 4361-4369.
Dentelli P, et al. (1999) J Immunol. 163: 2151-2159.
Meinke A, et al. (1996) Mol Cell Biol. 16: 6937-6944.
P-Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using STAT5A (phospho-Ser780) antibody.
Western blot analysis of extracts from HeLa cells using STAT5A (phospho-Ser780) antibody.