• Catalog Number:

  E1A8325A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CtBP1 around the phosphorylation site of serine 422 (A-P-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13363

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  C-terminal binding protein 1; CTBP; EC 1.1.1.-

• Specificity Sensitivity:

  CtBP1 (Phospho-Ser422) antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422.

• References:

  Schaeper U., Proc. Natl. Acad. Sci. U.S.A. 92:10467-10471(1995). Sewalt R.G.A.B., Mol. Cell. Biol. 19:777-787(1999). The MGC Project Team, Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser422; Mouse: Ser423; Rat: Ser412

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using CtBP1 (Phospho-Ser422) antibody.