• Catalog Number:

  E1A1242A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P08670

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  VIME

• Specificity Sensitivity:

  Vimentin (Phospho-Ser83) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 83.

• References:

  Ferrari S., Mol. Cell. Biol. 6:3614-3620(1986). Honore B., Nucleic Acids Res. 18:6692-6692(1990). Hartmann T.B., Br. J. Dermatol. 150:252-258(2004).

• All Sites:

  Human: Ser83; Mouse: Ser83; Rat: Ser83

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho-Ser83) antibody. Western blot analysis of extracts from 293 cells, treated with paclitaxel (1uM, 24hours), using Vimentin (Phospho-Ser83) antibody.