• Catalog Number:

  E1A8158A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of serine 204 (T-R-SP-V-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  Q13153

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  ADRB2; alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; p21-activated kinase 1; P65-PAK; P68-PAK; PAK 1; PAK-1; PAKA; protein kinase MUK2

• Specificity Sensitivity:

  PAK1 (Phospho-Ser204) antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204.

• References:

  Brown J.L.,Curr. Biol. 6:598-605(1996). Sells M.A.,Curr. Biol. 7:202-210(1997). The MGC Project Team;Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser204; Mouse: Ser204; Rat: Ser203

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody. Western blot analysis of extracts from 3T3 cells, treated with UV (15mins), using PAK1 (Phospho-Ser204) antibody.