• Catalog Number:

  E1A7014A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of Threonine 69 or 51.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15336

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  ATF-2(Phospho-Thr69 or 51) Antibody detects endogenous levels of ATF-2 only when phosphorylated at Threonine 69 or 51.

• References:

  Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465. Alsayed Y, et al. (2001) J Biol Chem. 276(6): 4012-4019. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393.

Western blot analysis of extracts from 3T3 cells, using ATF-2 (Phospho-Thr69 or 51) Antibody.

Western blot analysis of extracts from LOVO cells. Line1: Using ATF-2 (Phospho-Thr69 or 51) Antibody; Line2: Using the same antibody preincubated with synthesized peptide.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF-2 (Phospho-Thr69 or 51) Antibody Antibody; Right: The same antibody preincubated with synthesized phospho-peptide.