• Catalog Number:

  E1A1154A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of serine 186 and serine 188 (H-K-SP-D-SP-I-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q00987

• Applications:

  IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  Double minute 2 protein, EC 6.3.2.-, Oncoprotein Mdm2, Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2

• Specificity Sensitivity:

  MDM2 (Phospho-Ser186+Ser188) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.

• References:

  M Zhou, Blood, Mar 1995; 85: 1608 - 1614. Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030. M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.

• All Sites:

  Human: Ser186+Ser188

P-peptide - +

Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) antibody. MDM2 (Phospho-Ser186+Ser188) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.