Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from humanα-Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P37840
Applications:
WB:1:500~1000
Specificity Sensitivity:
α-Synuclein (phospho-Ser129) antibody detects endogenous levels ofα-Synuclein only when phosphorylated at serine 129.
References:
Jakowec MW,et al.(2001)Dev Neurosci.23(2):91-9.
Kitamura Y, et al.(2001)Brain Res.898(1):181-5.
El-Agnaf OM,et al.(2006)FASEB J.20(3):419-25.
Western blot analysis of extract from mouse brain tissue, usingα-Synuclein (phospho-Ser129) antibody (E011171).