• Catalog Number:

  E1A0011D-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human human Histone H4 around the acetylation site of lysine 5(R-G-KA-G-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  P62805

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  H4F2, HIST, HIST1H4A, Histone H4

• Specificity Sensitivity:

  Histone H4 (Acetyl-Lys5) antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine 5.

• References:

  M Couppez, J. Biol. Chem., Feb 1987; 262: 2854 - 2860. BM Turner, J. Cell Sci., Jun 1990; 96: 335. Albig W., Genomics 10:940-948(1991).

• All Sites:

  Human: Lys5; Mouse: Lys5; Rat: Lys5

A-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody.

A-peptide - + Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody.