Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CDC37 around the phosphorylation site of serine 13 (E-V-SP-D-D).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q16543
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
CC37, Hsp90 chaperone protein kinase-targeting subunit, Hsp90 co-chaperone Cdc37, p50Cdc37
Specificity Sensitivity:
CDC37 (Phospho-Ser13) antibody detects endogenous levels of CDC37 only when phosphorylated at serine 13.
References:
Stepanova L., Genes Dev. 10:1491-1502(1996).
Dai K., J. Biol. Chem. 271:22030-22034(1996).
Rao J., J. Biol. Chem. 276:5814-5820(2001).
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All Sites:
Human: Ser13; Mouse: Ser13 Rat: Ser13
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using CDC37 (Phospho-Ser13) antibody.
Western blot analysis of extracts from NIH/3T3 cells, using CDC37 (Phospho-Ser13) antibody.