• Catalog Number:

  E1A7099A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q92769

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  HD2; histone deacetylase 2; YY1 transcription factor binding protein; YY1BP

• Specificity Sensitivity:

  HDAC2 (Phospho-Ser394) antibody detects endogenous levels of HDAC2 only when phosphorylated at serine 394.

• References:

  Yang W.-M., Proc. Natl. Acad. Sci. U.S.A. 93:12845-12850(1996). Mungall A.J., Nature 425:805-811(2003). Schmidt D.R., Biochemistry 38:14711-14717(1999).

• All Sites:

  Human: Ser394; Mouse: Ser394

Western blot analysis of extracts from 293 cells, COS cells and HeLa cells, all treated with PMA (125ng/ml, 30mins), using HDAC2 (Phospho-Ser394) antibody.