• Catalog Number:

  E1A8433A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TIF-IA around the phosphorylation site of serine 649 (Q-P-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9NYV6

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  RNA polymerase I-specific transcription initiation factor RRN3; RRN3; transcription initiation factor IA

• Specificity Sensitivity:

  TIF-IA (Phospho-Ser649) antibody detects endogenous levels of TIF-IA only when phosphorylated at serine 649.

• References:

  Bodem J., EMBO Rep. 1-171-175(2000). Moorefield B., Proc. Natl. Acad. Sci. U.S.A. 97:4724-4729(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser649

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using TIF-IA (Phospho-Ser649) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using TIF-IA (Phospho-Ser649) antibody.