Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MER/SKY around the phosphorylation site of tyrosine 749/681 (K-I-YP-S-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
MER/SKY (Ab-749/681) antibody detects endogenous levels of total MER/SKY protein.
References:
Graham D.K., Cell Growth Differ. 5:647-657(1994).
Graham D.K., Cell Growth Differ. 5:1022-1022(1994).
Crosier K.E.,Growth Factors 11:137-144(1994).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using using MER/SKY (Ab-749/681) antibody.
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Ab-749/681) antibody.