Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P38398
Applications:
IHC: 1:50~1:100 ELISA: 1:4000
Alter Name:
BRCA1, Breast cancer type 1 susceptibility protein
Specificity Sensitivity:
BRCA1 (Ab-1423) antibody detects endogenous levels of total BRCA1 protein.
References:
Kim ST, et al. (2002) Genes Dev; 16(5): 560-570.
Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709.
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718.
Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46.
Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Ab-1423) antibody (#B7030).