• Catalog Number:

  E1A7012A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of Serine 112 or 94 (D-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15336

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  ATF-2 (phospho-Ser112 or 94) antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 112 or 94.

• References:

  Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (phospho-Ser112 or 94) antibody.

Western blot analysis of extracts from MDA-MB-435 cells using ATF-2 (phospho-Ser112 or 94) antibody.